nonvoting denebola: News and Society -Crime News Blog: Keeping ...

ByAndrew Keel

Most people tend to think of jail as a place that criminals are put so that society as a whole will be safe. We want to think that every person who is put in the county jail is guilty of some kind of crime. What so many of us lose sight of is that when you have a large number of people living in cramped quarters, there are bound to be problems. Since the average county jail is currently over crowded or near its capacity to house prisoners, more and more concerns about inmate safety are being raised.

One of the reasons that some people are becoming more vocal about the subject of county jail safety is because some of the inmates have serious mental illnesses. Some of the inmates in the county jail could suffer from psychosis, schizophrenia, bipolar, as well as minor cognitive disabilities. Not only are people concerned about what will happen to the individuals who are suffering from the metal illnesses when they are placed in custody, but there is serious concern about how having these individuals in the jail could compromise the safety other individuals that are in full control of their mental state.

The truth is that in some situations it might actually be better for everyone if individuals who are struggling with illnesses like schizophrenia and bipolar to be placed in a state prison that has better funding resources and adequately trained professionals. The average county jail simply doesn't have the space or the man power to give these inmates the care and attention that they need. The larger prisons have psychologists who can regularly see the prisoners and gauge their state of mind. There will also be guards who are going to be specifically educated to keep an eye on the prisoners and proactively handle situations before they have a chance to get out of hand.

If someone you know has a mental illness and has recently been incarcerated in the county jail, it is going to be up to you to keep an eye on them. Inmate locators will make it possible for you to gather all of the information you need about where they are, their arrest, bail, and what their visitation schedule is going to be. Once you have confirmed the location of the county jail where they're incarcerated, you need to contact the jail and alert them to the situation including treatment plans, medications and, and the names of any healthcare providers that can be contacted to procure copies of medical records. The sooner everyone knows about the inmates mental issues, the sooner they can take the steps to make sure that everyone is safe.

Another way to support an inmate is simply by staying in touch. If you find it stressful to write a letter and mail it by hand, you can email an inmate from the web or your smartphone.

Article Source:http://EzineArticles.com/?expert

Source: http://newsandsociety-crimenews.blogspot.com/2012/07/keeping-inmates-and-mentally-ill-safe.html

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Source: http://nonvoting-denebola.blogspot.com/2012/07/news-and-society-crime-news-blog.html

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Find The Stock Market Confusing? Keep Reading

[unable to retrieve full-text content]It is very exciting to jump into the stock market. There are many different investment vehicles, tailored to different financial goals and involving different amounts of risk. No matter which stocks strike your interest or how much ...

Source: http://www.compoundedknowledge.com/find-the-stock-market-confusing-keep-reading/

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Sunday's York-Adams sports scoreboard

Following is Sunday's schedule of York-Adams sports events. Results will be posted as they become available.

AMERICAN LEGION BASEBALL

Region 4 Tournament

Spring Grove 11, Campbelltown 7, F

York-Adams All-Star Game

American 9, National 8, F

SUSQUEHANNA LEAGUE BASEBALL

Windsor 6, Felton 3, F.

Columbia 13, Stewartstown 1, F

BIG 26 BASEBALL CLASSIC

Maryland 4, Pennsylvania 3, F

ATLANTIC LEAGUE BASEBALL

York 8, Camden 6, F

LOCAL GOLF

YORK COUNTY AMATEUR

T.J. Ostrom (74-67-141) wins by three shots over Gary Stewart Jr. (70-74-144).

Source: http://www.yorkdispatch.com/sports/ci_21133201/sundays-york-adams-sports-scoreboard?source=rss

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I HATE when people / OHs family - BabyandBump

I know, people do that Everyone has different associations and experiences and beliefs about different names, and no one name will ever be agreed on by everybody. I just think you totally ignore them and pick a name you and your OH love and think fits your little one Even if they hate the name initially, once they get to know your little baby those ideas go out the window, and then your family couldn't imagine him/her with a different name
Personally, I dislike everyone asking us about names right now. My DH has made it very clear he doesn't want to talk about names until the baby is here, which to me is stressful! We've been arguing about it (finally both did some compromising), so I hate feeling that pressure from people asking what we're going to name him! It's an emotional topic for me right now, haha, so I feel like I have to hold back when people ask! lol

Source: http://babyandbump.momtastic.com/pregnancy-second-trimester/1106071-hate-people-ohs-family.html

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Should Hector Lombard drop to welterweight after debut loss at UFC 149?

Hector Lombard came into UFC 149 riding a long win streak and an unstoppable hype train. He walked out of UFC 149 with a decision loss and a disappointed boss.

"It wasn't the fight I was expecting," UFC president Dana White admitted. "It just wasn't what I thought it was going to be. Whether it was Boetsch or Lombard winning, I was expecting a real war. I thought this thing was going to be a war."

It was far from an exciting bout, as Lombard, known for his aggression and knockouts, showed little aggressiveness in the bout. White wondered if Octagon jitters got the best of Lombard, and also threw out the idea that middleweight is too big of a weight class.

"Lombard maybe should fight at 170," White said. "He made 185 easily. He's short and wide, but I think he could make 170."

In a Q and A before the UFC 149 weigh-ins, middleweight Michael Bisping made jokes about Lombard's height.

"I bumped into Hector Lombard in the elevator, and I thought someone was playing a practical joke. I was like, 'Who is this dwarf?' I think he's in the wrong weight class. I think he's in the 125 weight class."

At 5-foot-9, Lombard is one of the shortest middleweights in the division. Most 185ers in the UFC are at least six-foot. Champion Anderson Silva is 6-foot-2. Though fighters have overcome height and reach differences in bouts -- think Pat Barry knocking out Stefan Struve -- there is no reason for Lombard to come into the fight at a disadvantage if he is comfortable dropping down.

Should he drop down? Tell us your thoughts in the comments, on Facebook or Twitter.

Other popular content on the Yahoo! network:
? Tim Tebow working with former MLB pitcher on his throwing mechanics
? Ernie Els comes from six shots back to win British Open in dramatic fashion
? Ronda Rousey calls out Michael Phelps for diva behavior in Beijing
? Y! Screen: Watch as a kitten attacks a doberman

Source: http://sports.yahoo.com/blogs/mma-cagewriter/hector-lombard-drop-welterweight-debut-loss-ufc-149-135354108--mma.html

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Foster parent contemplating domestic adoption - Adoption.com ...

Hi Everyone,

I have been a foster parent for 3 and a half years. I adopted my first placement, and since then I've only had 2 other placements, both of which were temporary. I enjoy providing foster care and want to continue being a foster parent. However, my daughter is 4 now and I feel very ready to adopt again.

I chose foster care originally because of financial reasons. Mainly - adopting from the state is very inexpensive and Social Services pays for the adoption. I am still not rich by any means, but I am in a better financial situation than I was when I started looking into adoption.

So, I am now contemplating the idea of domestic infant adoption, and I have plenty of questions. First of all, while I have been able to foster/adopt as a single parent, I have felt slightly discriminated against. I got my placements when all "couples" homes were full or not taking placements. I've had the least placements of my group in DSS. So, I'm just wondering how far that discrimination runs - have any of you felt like you had less of a chance of adopting, because of your family dynamics?

I want to ask opinions on adoption agencies as well, but I'm unclear about whether you need to use a local agency or you can use one out of state? Either way, are you presented to birth mothers only with your local area, or anywhere within the US?

With that said, I'd love to hear from some of you about the agencies you used and whether you would recommend them or not. I know some agencies seem to work better with singles than others do.

Any and all advice is welcome. I'm not brand new to adoption, but I am brand new to domestic adoption and would love to learn as much as I can!

Thank you!

Source: http://forums.adoption.com/single-adoptive-parent-support/405921-foster-parent-contemplating-domestic-adoption.html

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Hulu Updates Video Player, Adds 10-Second Rewind And Easier-To-Use Settings

Hulu released a new, chromeless video player on Saturday, in an effort to improve the viewing experience for users of its web site. The new player is designed to give viewers easier access to their settings, and has also added a 10-second rewind feature to enable viewers to quickly skip back and re-watch their favorite moments of a program. The new player groups all of a video's settings -- facial recognition, closed captioning, and the like -- all in one place, and the player automatically detects and adjusts video quality based on the available bandwidth. And when you pause a video, the player highlights program and episode information, along with how much time is left in the show or movie you're watching.

Source: http://feedproxy.google.com/~r/Techcrunch/~3/GtiOjdoJkjs/

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Syrian forces bombard Damascus, fight rages in Aleppo

BEIRUT/AMMAN (Reuters) - Syrian forces bombarded three districts of Damascus with helicopter gunships on Sunday, witnesses said, clawing back territory from rebels a week after the fighters launched what they called a final battle for the capital.

Fighting also raged around the main intelligence headquarters in Syria's biggest city, Aleppo -- the country's main commercial and industrial hub -- and in Deir al-Zor on the Euprhates river, the largest city in the east.

Rebels said they had captured a third border crossing with Turkey on Sunday, Bab al-Salam north of Aleppo, while Iraqi officials said Syrian forces had regained control of one of two border crossings seized by rebels on the frontier with Iraq.

The helicopter bombardments in Damascus and Deir al-Zor showed President Bashar al-Assad's determination to regain control after a bomb killed four members of his high command in the gravest blow in the 16-month-old revolt.

Rebels were driven from Mezze, the diplomatic district of Damascus, residents and opposition activists said, and elite Fourth Division troops were besieging the northern neighbourhood of Barzeh, one of three northern areas hit by helicopter fire.

The fourth division is run by Assad's younger brother, Maher al-Assad, 41, who is widely seen as the muscle maintaining the Assad family's four decades of Alawite minority rule.

His role has become more crucial since Assad's defence and intelligence ministers, a top general and his powerful brother-in-law were killed by the bomb on Wednesday, part of a "Damascus volcano" by rebels seeking to turn the tables in a revolt inspired by Arab Spring uprisings in Tunisia, Libya and Egypt.

Assad has not spoken in public since the bombing. Diplomats and opposition sources said government forces were focusing on strategic centres, with one Western diplomat comparing Assad to a doctor "abandoning the patient's limbs to save the organs".

Syrian state television quoted a media source denying that helicopters had fired on the capital. "The situation in Damascus is normal, but the security forces are pursuing the remnants of the terrorists in some streets," it said.

Assad's forces, who also pushed into a rebel-held district in the northerly commercial hub of Aleppo on Saturday, targeted pockets of lightly armed rebels, who moved about the streets on foot and attacked security installations and roadblocks.

Residents said the sound of shelling in the capital was so intense at dusk that they were unable to distinguish it from the traditional cannon blast marking the end of the daily fast for the Muslim holy month of Ramadan.

Opposition activists said late on Saturday that helicopters had fired rockets into a neighbourhood near the southerly Sayida Zeinab district, causing dozens of casualties. They did not have any other details.

"In Damascus, people continue to search desperately for safety," the International Committee of the Red Cross (ICRC) said in a statement in Geneva.

"Humanitarian needs are growing as the situation in the city worsens and as large numbers of people flee their neighbourhoods in search of safe haven. The ICRC and the Syrian Arab Red Crescent have intensified their response to the situation."

The Syrian Observatory for Human Rights, an opposition group that monitors the violence, said 180 people, including 48 troops, had been killed across Syria on Saturday. Many of them died in the province of Homs, epicentre of the uprising.

Most shops in Damascus were closed and there was only light traffic - although more than in the past few days. Some police checkpoints, abandoned earlier in the week, were manned again.

Many petrol stations were closed, having run out of fuel, and those that were open had huge lines of cars waiting to fill up. Residents reported long queues at bakeries.

FLIGHT FROM ALEPPO

A bloody crackdown on what began as a peaceful revolt has increasingly become an armed conflict between an establishment dominated by Assad's Alawite minority, an offshoot of Shi'ite Islam, and rebels drawn largely from the Sunni majority.

U.N. Secretary General Ban Ki-moon said he was sending his peacekeeping chief Herve Ladsous and top military adviser General Babacar Gaye to Syria to assess the situation.

Opposition activists in Aleppo said hundreds of families were fleeing residential areas on Saturday after the military swept into the Saladin district, which had been in rebel hands for two days.

Fighting was also reported in the densely-populated, poor neighbourhood of al-Sakhour.

"The sound of bombardment has been non-stop since last night. For the first time we feel Aleppo has turned into a battle zone," a housewife, who declined to be named, said by phone from the city.

REBEL BORDER CROSSING RAID

On the Iraqi-Syrian border, Iraqi security and border officials said Syrian forces had reasserted control over the Yarubiya crossing point on the Syrian side of the frontier, briefly seized by rebels on Saturday.

Syrian opposition activists said several towns in Syria's Kurdish northeast had passed - without a fight - into local hands in recent days as central authority eroded.

The surge in violence has trapped millions of Syrians, turned sections of Damascus into ghost areas, and sent tens of thousands of refugees fleeing to neighbouring Lebanon.

The U.N. Security Council has approved a 30-day extension for a ceasefire observer mission, but Ban has recommended changing its focus to pursuing prospects for a political solution - effectively accepting there is no truce to monitor.

Diplomats said only half of the 300 unarmed observers would be needed for Ban's suggested plan, and several monitors were seen departing from Damascus on Saturday.

Speaking two days after Russia and China vetoed a resolution to impose U.N. sanctions on Assad's government, Ban called on he Security Council to "redouble efforts to forge a united way forward and exercise its collective responsibility".

"The Syrian government has manifestly failed to protect civilians and the international community has collective responsibility to live up to the U.N. Charter and act on its principles," he said.

Regional and Western powers have voiced concern the conflict might become a full-blown sectarian war that could spill across borders. But Assad's opponents remain outgunned and divided.

French Foreign Minister Laurent Fabius, speaking after contacts with the head of the Arab League and Qatar's prime minister, said all three agreed that it was time for Syria's fractured opposition to prepare to take charge of the country.

"We would like to see the rapid formation of a provisional government representing the diversity of Syrian society," said Fabius. Syria's main political opposition group, the Syrian National Council, operating in exile, has so far failed to unite Assad's disparate foes on a united political platform.

Source: http://news.yahoo.com/syrian-forces-bombard-damascus-fight-rages-aleppo-112830920.html

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Americare home health - HEALTH, BEAUTY & FITNESS

These pointers and extra information If only is going to be of gain to everybody. William Li, suggested adding hard cheeses like Gouda for your diet. Therefore purchasing one on your own may not provide you with a great problem. Hartz Flea & Tick control is essential for that health insurance and well- being of dogs and humans alike. The debate surrounding adding this group towards health article the healthcare plan has related to the truth that this can be a high risk group. The 24- month intensive program includes classes on weekends and prepares students for careers in hospital administration, pharmaceutical drugs, residential care or research facilities. To locate the body mass you can turn to the web and discover a Body mass index ( bmi calculator. About GizmoHealth. com We of experienced, devoted and diabetes fully licensed agents are prepared willing and able to assist insurance consumers navigate the insurance coverage process health to obtain the right insurance policy for their coverage needs and budgets. How can you would like your health to become? The quantity of tonic water an individual will have to consume every day to be able to experience any significant health advantages in the quinine is 1. on 77 liters or 60 fluid oz .. It? s that a part of an Auckland Plumber job that americare creates a natural or artificial elimination of water from surface and sub- surface. M. Most significantly, never choose forced feed for the kid. Any adverse health insurance for individuals who? ve families can help purchase prescription medicine for kids who become ill or hurt. It practically enhances your posture. For additional on healthcare americare home health administration undergraduate degree programs,, visit our overall health and medicine section. This supplement consists of similar elements towards the earlier pointed out item, but is provided inside a more appealing berry flavor. Research signifies a potential outcomes of an excess of the natural fungus in your scalp and dry skin. This clinic provides free healthcare Simply to without insurance and underinsured recorded entertainment industry professionals between your age range of 18 to 64. The very first strategy is fundamental, but something on everyone? s mind and that is price of care. Discount rates were extremely truly provided to the holders from the medical health insurance plans and all sorts of this method in general home labored really perfectly as well as americare home health become very famous. It is sometimes less expensive to insure each member of the family individually, especially children, since each individual may require their very own particular degree of health article on diabetes coverage.

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So in while travelling an individual will get hurt, it won? t be incorporated within the simple medical health insurance plan. By using healthcare The next information consists of a couple of tips about how to correctly use vitamins. When the thoughts are upset it might modify the 4. Health Advantages Of D- Ribose D- Ribose is really a dietary supplement that? s been around for any very long time but became Health insurance plan isn? t just for a person only one can insure the healthiness of their family people too. You will find many When it comes to healthiness, although we now have be vulnerable, it appears we don? t have just as much time at our disposal

Source: http://curryrecipe-jp.net/health-2/americare-home-health/

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Iridium Satellite Phone Service Covers The Globe | welcome to ...

Providing govt, business and residential customers with the strength of satellite tv for pc driven marketing and sales communications, the Iridium satellite tv for pc phone service is probably the industry leaders in using fractional treatments. The company will be noted beeing the service provider for the United States Division of Safeguard, which goes wrong with speak properly for the advances that have been stated in satellite marketing and sales communications technology within the last few decades.

Instead of rely on giant dishes and impossible to make use of devices, the likes of Iridium satellite voice service have managed to shrink the technology to more usable forms. The satellite tv for pc phones being used today often operate and appearance more like regular land lines or perhaps cell phones, however the power that backside them is much greater.

The Iridium satellite telephone company, for example, operates using about Sixty six low-earth orbiting satellite. These devices are cross-linked to provide because seamless of coverage as possible for customers that are fairly broad varying. Thanks to it?s massive satellite tv for pc installation, Iridium is definitely the owner of the greatest satellite constellation inside entire world. The business tends to serve business and government clientele, but its software are also popular with many residential customers who reside in out of the way areas.

As the Iridium system becomes more audio and dependable, more businesses and even residential customers are looking at this company to provide their voice and data transfer solutions. Industries that benefit from satellite voice service, include:

Safeguard: When telephone lines go lower, satellites don?t. This is the reason the reason why government agencies such as the Department of Defense rely on companies like Iridium. It isn?t really a good idea for the defense office to lose exposure to its crucial employees. Spoutnik help make confident this doesn?t happen.

Transport: Many seafaring companies rely on satellite mobile phones for their insurance areas and convenience. Certainly not relying on cell towers to power these, these phones are ideal for shipping companies that have yachts traveling in and out of distant regions.

Flight handling: There aren?t land lines on aircraft, but there are satellite mobile phones. These devices work above and beyond regular radio frequencies to keep passengers and pilots in touch with the floor. Whether it?s commercial aviation or government linked, satellite mobile phones are quickly the interaction tool associated with preference.

Transportation: We?ve all heard the actual horror stories concerning going through the mountains and losing cell phone program. Transportation companies that rely on satellite tv for pc phone services, such as Iridium, do this to prevent this particular eventuality. Whenever staying in regular touch is very important, companies such as the Iridium satellite voice service are a good way to travel.

Emergency services: Agencies offering emergency and humanitarian services in distant locations are turning to satellite tv for pc phone service to be sure their worker have regular contact with the other person and their home offices. Because satellite mobile phones can offer virtual worldwide insurance, they provide a sound solution where the might be hardly anything else.

As technological innovation marches forward also does the actual reliability and effectiveness of satellite technological innovation. Powering marketing and sales communications for govt and market, the satellite tv for pc phone services provided by the likes of Iridium are become the way company gets completed.

Christopher Cruz have been a masterful psychologist for over 16 yrs and has studying excellent ideas in iridium9575 as part with her involvement from Creative Ideas Team ,a new creative team for creative people. Find out about her website to read more about her iridium advice over the years.

Source: http://www.nwactc.net/iridium-satellite-phone-service-covers-the-globe/

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A Golden Age for Lithium-Air EV Batteries?

Charging the Ford Focus EV, one of the current crop of electric vehicles running on Lithium-ion. Credit: Ford

Lithium-ion batteries have become standard for portable electronics because they don?t require a lot of power to run. However, using Li-ion batteries for bigger purposes, like powering an electric car, does not bode as well. Many of the newest electric vehicles can run for only 100 miles (if that) before needing a recharge.

Among the many alternative technologies researchers are investigating is lithium-air, a way to build batteries with a much higher energy density than Li-ion. This week in the journal Science, a group of chemists at the University of St Andrews in Scotland led by Peter Bruce reports on a prototype battery that can more efficiently use oxygen in the atmosphere as a source of power. All it takes is a little gold.

The idea of lithium air is that it could gather the oxygen it requires from the atmosphere, which would allow the battery to be lighter because it wouldn?t need to store heavy metal oxides. The reactions inside the battery would form lithium peroxide while the battery was expending energy, and, in theory, the opposite reaction would happen while the battery was charging, releasing oxygen back out into the air.

But in most prototypes so far, which used carbon-based electrodes, the battery didn?t stand up to repeated discharging and charging cycles, due in part to unwanted reactions happening on the side. That degredation meant the battery wasn?t fully rechargeable. But by making the electrodes out of gold (and adding in some dimethyl sulfoxide), Bruce and colleagues got rid of these side reactions almost entirely. Once all the lithium ions have been used up, all it takes is a flick of a switch to reverse the reaction and make them available once again. Even after 100 charging cycles, the battery retained 95 percent capacity.

Bruce and his colleagues acknowledge that car batteries with electrodes of pure gold don?t make much economic sense. However, he puts forth the idea that it?s possible to take a carbon electrode and thinly coat it in gold to achieve the same results.

Source: http://www.popularmechanics.com/how-to/blog/a-golden-age-for-lithium-air-ev-batteries-10851936?src=rss

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Bulgaria official: Blast suspect had fake Michigan ID

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Source: http://news.yahoo.com/bulgaria-official-blast-suspect-had-fake-michigan-drivers-090650897--abc-news-topstories.html

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Auto Insurance Converse In Today's Entire world. Nothing But The ...

Some websites offer you car insurance info. You can easily get lost inside an ocean Converse All Star of which should you be not cautious. Selecting by way of everything to locate info which is relevant to you may not be fun, or fast. Fortunately, there are actually everything you should know here. Continue reading to get more.

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And also hardwearing . car insurance premiums to a minimum, decrease your want to only extensive insurance when a car or automobiles are parked. In case you are not at the moment driving a car but nonetheless desire to make it protected against wanton damage or damages related to disasters, then you only need comprehensive insurance coverage.

Very careful, some vehicle insurance insurance policies usually do not protect harm carried out while you are driving a vehicle other engine autos, including motorcycles, a number of wheelers, snowmobiles, or jet skis. Be sure you look at the policy to find out whether or not these autos are taken care of before you decide to Converse All Star journey in one, or at least, ensure your medical health insurance provides protection to you.

Verify the accident score and also the robbery ranking for any new auto you are considering buying. Automobile insurance organizations cost much more for insurance policies on vehicles they feel are at an increased chance of simply being robbed, or who have a negative collision score in comparison to other vehicles with far better reviews.

Discounts on car insurance insurance are sometimes supplied for reduced miles individuals. Should you only travel a couple of a long way daily to get to operate, or if you take public transportation, you will likely be eligible for a minimal miles discount on your plan. Be sure you Converse All Star question your representative prior to finalize your car insurance program.

In case your auto is covered with insurance with a number of individuals and one halts making use of the automobile, inform your insurance firm instantly. This is basically the genuine course of action. Furthermore, it can lessen your monthly premiums significantly in many cases. Fresh drivers, older individuals, and drivers with terrible data all enhance your top quality. Have them pulled from your plan when you can.

When you are just starting out generate, ensure you are over a policy by having an more aged, more capable driver. Many car insurance firms provide a reduce price when a youthful driver is paired up on the very same plan as being an more aged one particular. When possible, it is a wonderful strategy for cutting your family?s Converse All Star auto insurance rate.

Sifting through a sea of data is tough, as reviewed at the outset of this post. As you now have see the great advice here, you can go forwards with certainty in regards to what you might have learned. Refer to this car insurance information anytime you have to invigorate the concepts talked about here.

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Source: http://answers.apostoliccm.com/2012/07/auto-insurance-converse-in-todays-entire-world-nothing-but-the-ideal-recommendations/

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Sony Vaio T13 review - Bonnie Cha - Product Reviews - AllThingsD

If you?re shopping for a new laptop, you might find the term ?Ultrabook? popping up a lot during your research. An Ultrabook is thinner and lighter than a regular laptop, but it doesn?t sacrifice such features as a full keyboard, and it uses a different kind of memory instead of traditional hard drives to offer speed and good battery life, so you can use it as your main computer for everyday tasks like browsing the Web and working on documents. After a trend started by Apple?s MacBook Air, Windows PC makers such as HP, Dell and Samsung offer them, and now Sony has joined the fray.

Over the past week, I?ve been testing the Sony Vaio T13, the company?s first Ultrabook, and what makes it stand out from the pack is its affordable price tag. The cost of Ultrabooks still hovers in the $1,000 range but the Vaio T series starts at $770. By comparison, the similarly featured Dell XPS 13 costs $999 and up, while the 13-inch MacBook Air starts at $1,199.

For the price, the Vaio T13 offers Intel?s latest Ivy Bridge processors, a plethora of ports and four gigabytes of memory. To keep costs down, there are some tradeoffs, such as a lower resolution screen, but overall, I found the Vaio T13 to be a speedy machine. It?s a good value to be sure, but there are some downsides, such as a subpar keyboard and loud fan, that holds it back from excellent.

The Vaio T13 measures 12.7 inches wide, 0.71 inch thick and weighs 3.54 pounds, so it?s heavier than a number of ultrabooks on the market. For example, the Dell XPS 13 is 2.99 pounds and the MacBook Air weighs 2.96 pounds. That said, I didn?t find the extra weight to be a huge problem. I was able to carry it around in my backpack without it weighing me down.

Also, while it?s thicker than the Air, I appreciated the selection of ports available on the Vaio. This includes an Ethernet jack, two USB ports, HDMI and VGA ports if you want to connect to a TV or monitor and a Memory Stick Pro Duo/SD card slot.

The Vaio T has a 13.3-inch display with a 1,366 by 768 pixel resolution. Colors looked a bit dull, and pictures and videos weren?t overly bright or sharp. The lower resolution also means you have to scroll a bit more to see what?s onscreen, but you do get a decent-size touchpad that supports multitouch gestures to help you navigate various pages and switch between tasks. It worked well in my tests as I was able to return to the desktop by swiping down on the touchpad with four fingers and move between Web pages and photos by swiping left or right with three fingers.

But I didn?t like the Ultrabook?s keyboard. There?s plenty of spacing between the keys, so it doesn?t feel cramped. But the buttons are stiff and squishy, so I really had to punch at the keys. It?s something I got used to after a while, but it led to a lot of mistakes and frustration at the beginning. I also wish the keyboard was backlit for easier typing in darker rooms.

The $799 Vaio T13 I reviewed is powered by an Intel Core i5 processor and features a combined 500GB hard drive and 32GB solid-state drive. (You can configure the ultrabook with a faster i7 processor and up to 8GB of memory.) A number of Ultrabooks feature only solid-state drives ? or flash memory ? that improve speed and battery life but limit storage capacity. They are also more expensive, which is why Sony used this hybrid option.

Even so, I found the Vaio T13 to be a responsive computer and you get the bonus of extra storage capacity. It took the Ultrabook 29 seconds to boot up from a cold start and seven seconds to wake up from sleep mode. The MacBook Air started up in 15 seconds and awoke in five seconds, so there isn?t that much of a difference in the latter test.

At one time, I had at least a dozen tabs open in my Web browser, streamed a YouTube video and downloaded an album from the Google Play Web store while working on a Word document. The Vaio T13 was able to handle all those tasks without slowing down. However, its fan does tend to make a lot of noise. It?s annoying, but I suppose it is doing its job, as the Ultrabook never got warm, even when I was viewing entire TV shows on Hulu.

Sony estimates the Vaio T13?s battery life to be around seven hours and 30 minutes with power-saving mode on. In my tests, where I turned off all power-saving features and left Wi-Fi on, set the display to full brightness and looped a music playlist while running an email application in the background, the Vaio delivered four hours and 20 minutes of battery life.

The battery performance is middle-of-the-road compared to some of the other Ultrabooks we?ve tested at AllThingsD, with the Dell XPS 13 being the worst at under four hours and the Lenovo IdeaPad U300s being the best at five hours. With more normal usage, I expect you should get about an additional hour of battery life from the Vaio T13.

The Vaio runs Windows 7 and comes preloaded with a starter edition of Microsoft Office 2010, which offers basic versions of Word and Excel, and a 30-day trial of Kaspersky Internet Security. There are also various Sony apps, such as the company?s Music Unlimited store and PlayMemories multimedia gallery ? all of which you can access from a pull-down menu at the top of the desktop screen.

For those on a budget, the Sony Vaio T13 is certainly worth a consideration. It?s fast and offers a good amount storage. But if possible, I?d definitely recommend going into a store and trying out the keyboard to see if it?s to your liking.

Source: http://allthingsd.com/20120719/sonys-first-ultrabook-targets-the-budget-conscious/

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ucsf: #Melanoma treatment shows promise at UCSF http://t.co/ms8FfdwO via @SFGate #Cancer #SkinCancer @StephanieMLee

Sorry, Readability was unable to parse this page for content.

Source: http://twitter.com/ucsf/statuses/225631229435842560

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Video: Romney impersonator discusses impersonation

Handicapping the commercial space race

Three rockets lead the pack in NASA's competition for commercial spaceships, and there are good reasons why Liberty should be the frontrunner. Part 3 of a five-part series on "Spaceflight in the 20-teens" by NBC News' Jay Barbree.

Source: http://www.msnbc.msn.com/id/3036697/vp/48232579#48232579

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Are consumers aware that they are drawn to the center when choosing products?

Tuesday, July 17, 2012

Consumers are more likely to select products located in the horizontal center of a display and may not make the best choices as a result, according to a new study in the Journal of Consumer Research.

"A close investigation of visual attention reveals that consumers do not accurately recall their choice process. Our findings emphasize the relationship between horizontal location, attention, and choice," write authors A. Selin Atalay (HEC Paris), H. Onur Bodur (Concordia University), and Dina Rasolofoarison (Aston Business School).

Many products are arranged horizontally. For example, rows of snack bars in a vending machine, bottles displaying the beer selection in a bar, or jars of peanut butter on a supermarket shelf. How does this influence which option a consumer will choose?

Using eye-tracking devices, the authors investigated how location influences choices for products as varied as vitamins, meal replacement bars, and energy drinks. Consumers had a tendency to increase their visual focus on the central option in the final five seconds prior to a decision and this determined which option they would choose. Consumers did not accurately recall their choice process and were not aware of any conscious visual focus.

Another study in a retail environment demonstrated that the centrally located item within a product category is chosen more often, even when it is not placed in the center of the shelf or visual field. Consumers would make better choices if they were aware that their attention usually focuses on the center.

"In the context of low involvement choice between frequently purchased products, when choosing between unfamiliar yet equivalent brands, the visual search process and consumer choice are biased toward centrally located options. Being unaware that our attention is focused on the center can lead to poor choices," the authors conclude.

###

University of Chicago Press Journals: http://www.journals.uchicago.edu

Thanks to University of Chicago Press Journals for this article.

This press release was posted to serve as a topic for discussion. Please comment below. We try our best to only post press releases that are associated with peer reviewed scientific literature. Critical discussions of the research are appreciated. If you need help finding a link to the original article, please contact us on twitter or via e-mail.

This press release has been viewed 28 time(s).

Source: http://www.labspaces.net/121778/Are_consumers_aware_that_they_are_drawn_to_the_center_when_choosing_products_

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Obesity plus low vitamin D may add up to a greater risk of diabetes

ScienceDaily (July 17, 2012) ? The combination of obesity and vitamin D deficiency may put people at even greater risk of insulin resistance than either factor alone, according to new research from the Drexel University School of Public Health recently published early online in the journal Diabetes Care. Insulin resistance is a major risk factor for Type 2 diabetes, a condition that affects 25.6 million adults and is the seventh leading cause of death in the United States.

"Vitamin D insufficiency and obesity are individual risk factors for insulin resistance and diabetes," said lead author Shaum Kabadi, a doctoral candidate in epidemiology at Drexel. "Our results suggest that the combination of these two factors increases the odds of insulin resistance to an even greater degree than would have been expected based on their individual contributions."

In the study, obese individuals who had healthy levels of vitamin D had insulin resistance almost 20 times more often than the overall study population. But in obese individuals whose serum vitamin D was low, insulin resistance was much higher: about 32 times more common than the average.

Senior author Dr. Longjian Liu, an associate professor in the School of Public Health, noted, "It's not clear whether obesity itself causes a low vitamin D level or if it's the other way around."

Vitamin D is stored in adipose fat tissues, making it unavailable for the body to use; as a result, people who are overweight are already more likely to have low levels of serum vitamin D. Vitamin D deficiency is associated with multiple health conditions including diabetes, cardiovascular diseases including stroke, depression, dementia and other conditions.

Kabadi, Liu and co-author Dr. Brian Lee, an assistant professor in the School of Public Health, analyzed data on serum vitamin D levels and indicators of insulin resistance and diabetes from 5,806 respondents to a major national health survey, the National Health and Nutrition Examination Survey (NHANES). This was the first study of the association between vitamin D and diabetes risk for obese patients using a large, nationally representative sample of adults. The survey reported data from individuals at a single point in time and was therefore unable to determine whether there is a cause-and-effect relationship among vitamin D, obesity and insulin resistance.

Further studies could indicate whether vitamin D supplements are effective at reducing the risk of insulin resistance and diabetes in obese individuals. If so, the authors noted that it would be an inexpensive and practical prevention strategy compared to the difficulty involved in healthy weight loss.

Liu said that vitamin D supplements may be useful for people who are overweight or obese to help control diabetes, but he cautioned that too much vitamin D can cause side effects such as weakness and fatigue. In addition, vitamin D deficiency is just one of many known risk factors associated with insulin resistance and diabetes. "Therefore, to control diabetes, we need to have comprehensive prevention strategies. For example, physical activity may be the most cost-effective way to control weight and subsequently to control conditions including diabetes and heart disease," Liu said.

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The above story is reprinted from materials provided by Drexel University.

Note: Materials may be edited for content and length. For further information, please contact the source cited above.

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Journal Reference:

1. S. M. Kabadi, B. K. Lee, L. Liu. Joint Effects of Obesity and Vitamin D Insufficiency on Insulin Resistance and Type 2 Diabetes: Results from the NHANES 2001-2006. Diabetes Care, 2012; DOI: 10.2337/dc12-0235

Note: If no author is given, the source is cited instead.

Disclaimer: This article is not intended to provide medical advice, diagnosis or treatment. Views expressed here do not necessarily reflect those of ScienceDaily or its staff.

Source: http://www.sciencedaily.com/releases/2012/07/120717162738.htm

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Open-Access of UK-Funded Science Papers Will Start in 2013

Image: Flickr/nic221

From Nature News

From April 2013, science papers must be made free to access within six months of publication if they come from work paid for by one of the United Kingdom?s seven government-funded grant agencies, the research councils, which together spend about ?2.8 billion (US$4.4 billion) each year on research.

The policy, announced this morning by the agencies? umbrella body Research Councils UK (RCUK), makes clear that researchers should shun science journals that don?t allow authors to follow this mandate.

Also this morning, the UK government formally welcomed the Finch report into open access (which it had commissioned). Its response makes clear that RCUK?s new policy is the driving force for change.

RCUK hasn?t said how it will sanction those who don?t comply. (Astrid Wissenberg, who chairs the RCUK Impact Group, tells Nature that it will be looking to push to ?75% compliance over a number of years?). But if it does rigorously enforce the policy, that will mark a dramatic shift for scientists, publishers and universities ? perhaps the most significant change on the ground since Britain?s science minister David Willetts began discussing how to improve access to research papers more than a year ago.

Open ambition

RCUK released a draft version of the policy in March, and the final version makes no important changes, as the agencies had received mostly supportive comments, says an RCUK spokesperson. In essence, it is similar to announcements from the UK?s Wellcome Trust, a major biomedical research charity. But the research councils? move will be more influential, as Wellcome spends only ?600 million each year. Work funded in part by the research councils counts, so the policy includes overseas researchers collaborating with British scientists.

The research councils have said since 2006 that they want research to made free as soon as possible after publication. The difference today is that they are firmly stating the six-month maximum delay and, most importantly, are announcing how they will take money out of research grants to pay for open access.

Science journals have two ways of complying with the policy. They can allow the final peer-reviewed version of a paper to be put into an online repository within six months. Alternatively, publishers may charge authors to make research papers open-access up front.

In the United Kingdom in 2010, authors paid for immediate open-access publication for some 5% of papers (known as ?gold? open access), and another 35% were put into repositories after first being behind a paywall (?green? open access) ? except that some of those were not the final peer-reviewed version of the paper. (The proportions also vary between disciplines, as you can see from this chart.)

For ?gold? open access, RCUK will pay institutions an annual block grant to support the charges. If government doesn?t give RCUK any more cash, the money required will come from existing grant funding; it?s been previously estimated at some 1?1.5% of research budgets. In turn, RCUK expects that institutions will set up and manage their own publication funds. That might mean that universities and researchers will begin to discuss where they can afford to publish.

Source: http://rss.sciam.com/click.phdo?i=c29539630ffc78f3576a6687b5b2316c

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Why Don't the Old Ways Work in Business and Customer Service Any

?Why don?t the old ways work with customers anymore?? ? I hear this question frequently when?I?m speaking?at a business event or starting?a new? customer service consulting engagement.

First off: in certain, important ways, the old ways do still work.? Let me explain. What makes you great at social media and online customer service are very often the same skills that made you great before Facebook (sorry:? The Facebook) was even invented. In other words, social media is a dramatic amplifier of the positive, and of the negative?and if what you do for customers in your business is positive enough (great product, great problem resolution process, consideration of the emotional aspect of every interaction with your customers), the word can get out on a much bigger scale than ever before.

But the context of our work has undeniably changed, because of the recent?changes that have swept through the world of commerce.?Changes of?which social media is only one. Since the advent of the internet, and, most specifically, the broad use of the Web starting in the mid-1990s, there?s been a dramatic transformation of the competitive landscape. The changes?brought on by?these new communication and distribution channels are in many ways revolutionary, and they?re causing disruptions akin to those of past revolutions.

For example, our sense of timeliness: What was plenty fast this time last year feels draggy now to the very same customers because of changing expectations brought by mobile technology, social media?induced restlessness, the incredible efficiency of vendors like Amazon.com, and other factors.

For a parallel, look at the changes of the mid-nineteenth century. During this period the stability of rural and village life was thrown into disarray due to a host of technological advances, including those making it possible to preserve and transport food. Customers could now purchase edibles from across the country or around the world: The farmer in New England who had been able to count on a captive local market for whatever would graze or grow in his stony fields was now competing against topsoil-rich Illinois and lamb-friendly New Zealand. The result was a mass abandonment of farms throughout the region.

The transformation was striking: Go for a walk in the woods of New Hampshire or Vermont and you?ll still see the proliferation of old stone walls and foundations that attest to the abandoned farms and homesteads of this era.

Or just remember your high school poetry. This New England exodus is the backstory of Robert Frost?s stuck-in-his-ways neighbor still trying to mend a fence: He doesn?t realize times have changed and the fence, at most, is now preventing runaway trees. There are no cattle to contain anymore.

You can?t afford to be similarly left behind by today?s transformational technologies. It?s crucial to invest brain cells, time, and money to keep up with what it takes to hold on to your customers, now that we?re all playing on a global, digitally connected field.

Source: http://socialmediatoday.com/micah-solomon/611361/why-don-t-old-ways-work-business-and-customer-service-anymore

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Exclusive: GSK nears deal to buy Human Genome - sources

[ [ [['Connery is an experienced stuntman', 2]], 'http://yhoo.it/KeQd0p', '[Slideshow: See photos taken on the way down]', ' ', '630', ' ', ' ', ], [ [['Connery is an experienced stuntman', 7]], ' http://yhoo.it/KpUoHO', '[Slideshow: Death-defying daredevils]', ' ', '630', ' ', ' ', ], [ [['know that we have confidence in', 3]], 'http://yhoo.it/LqYjAX ', '[Related: The Secret Service guide to Cartagena]', ' ', '630', ' ', ' ', ], [ [['We picked up this other dog and', 5]], 'http://yhoo.it/JUSxvi', '[Related: 8 common dog fears, how to calm them]', ' ', '630', ' ', ' ', ], [ [['accused of running a fake hepatitis B', 5]], 'http://bit.ly/JnoJYN', '[Related: Did WH share raid details with filmmakers?]', ' ', '630', ' ', ' ', ], [ [['accused of running a fake hepatitis B', 3]], 'http://bit.ly/KoKiqJ', '[Factbox: AQAP, al-Qaeda in Yemen]', ' ', '630', ' ', ' ', ], [ [['have my contacts on or glasses', 3]], 'http://abcn.ws/KTE5AZ', '[Related: Should the murder charge be dropped?]', ' ', '630', ' ', ' ', ], [ [['have made this nation great as Sarah Palin', 5]], 'http://yhoo.it/JD7nlD', '[Related: Bristol Palin reality show debuts June 19]', ' ', '630', ' ', ' ', ], [ [['have made this nation great as Sarah Palin', 1]], 'http://bit.ly/JRPFRO', '[Related: McCain adviser who vetted Palin weighs in on VP race]', ' ', '630', ' ', ' ', ], [ [['A JetBlue flight from New York to Las Vegas', 3]], 'http://yhoo.it/GV9zpj', '[Related: View photos of the JetBlue plane in Amarillo]', ' ', '630', ' ', ' ', ], [ [['the 28-year-old neighborhood watchman who shot and killed', 15]], 'http://news.yahoo.com/photos/white-house-stays-out-of-teen-s-killing-slideshow/', 'Click image to see more photos', 'http://l.yimg.com/cv/ip/ap/default/120411/martinzimmermen.jpg', '630', ' ', 'AP', ], [ [['He was in shock and still strapped to his seat', 6]], 'http://news.yahoo.com/photos/navy-jet-crashes-in-virginia-slideshow/', 'Click image to see more photos', 'http://l.yimg.com/cv/ip/ap/default/120406/jet_ap.jpg', '630', ' ', 'AP', ], [ [['xxxxxxxxxxxx', 11]], 'http://news.yahoo.com/photos/russian-grannies-win-bid-to-sing-at-eurovision-1331223625-slideshow/', 'Click image to see more photos', 'http://l.yimg.com/a/p/us/news/editorial/1/56/156d92f2760dcd3e75bcd649a8b85fcf.jpeg', '500', ' ', 'AP', ] ]

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Source: http://news.yahoo.com/exclusive-human-genome-agrees-glaxosmithkline-takeover-sources-014610961--finance.html

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What Is Adware And Malware?

Many buyers affiliate spyware by technique of viruses. While it is true that much spyware shares comparable traits with viruses, there?s a central difference. Viruses had been initially created to destroy the computer?s capacity to work. Later on, they became the device by which on the internet thieves could steal identities. In virtually all situations, the proprietor of the computer was conscious that a major problem existed. All that modified through adware and malware.

The issues is Adware and Malware?

Adware is a quantity of because it attempts toward accomplish among the identical objectives of a virus without being detected; it?s more subtle. While adware does not seek toward destroy the pc?s functionality, it might collect information about you. Once more, even the data could also be less dangerous than that obligatory to steal your identity. Actually, a lot spyware and adware works toward secretly ship advertising to your laptop and set up toolbars.

Why do Buyers Build Spyware and adware and Malware Packages?

Why would any computer programmer want toward secretly install toolbars and deliver advertising as a substitute of stealing your identification? Effectively, it is very easy. The motivation continues to be profit. However, hackers are getting smarter regarding how they profit. They know that destroying your credit score and stealing your identification not only comes with heavy penalties including jail time, nevertheless it is also tantamount toward killing the goose that laid the golden egg.

As a substitute of completely targeting one specific and wiping out their finances, newer hackers are spreading spyware and adware that pays them a small amount, usually only a few cents, meant for each commercial delivered. Spyware and adware and malware is less complicated to ship to sufferer computers because largely end users do not protect from spyware and malware. That technique the hackers can revenue with spreading the malicious computer software over many extra computers.

The Effects of Spyware and adware on Your Laptop:

It?s possible you?ll be tempted toward say, ?Hey, as lengthy as they don?t seem to be bothering me, and it is not coming from my personal funds, then why ought to I care?? Well, it is true that the largest part malware and spyware will not utterly destroy your computer. On the additional hand, your laptop can grow to be very crippled without spyware and adware protection. You?ll notice the results at primary as a basic slowdown in all the things you do. What time an as soon as, your pc may not reply in favor of long intervals of time. It could even crash your laptop over and over. Essentially, spyware makes it so you?ll be able to?t effectively charge your pc as a result of the spy ware is utilizing it instead.

Spyware and adware Safekeeping and Safekeeping:

So as to defend from adware and malware, you need to carry a program particularly made used for catching and defending your PC from spyware and an assortment of harmful malware. When nearly all anti-virus package detect some spyware, they won?t catch all of it because they?re more particularly focused on viruses (which requires a barely several technique). However a dedicated spy ware application will give you full spy ware and malware safety. Check out PC Spyware and adware Safety made by Atanium Computer software which has a wonderful anti adware manufactured goods spyware security and safekeeping named PC Spyware and adware Safety.

Click here to read more about STOPzilla and STOPzilla Virus Removal.

Source: http://www.short-articles.net/what-is-adware-and-malware-3/?utm_source=rss&utm_medium=rss&utm_campaign=what-is-adware-and-malware-3

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Chomping At Bits: Florida Gators Offseason Special To Air Sunday

Chomping At Bits comes stocked with the best Florida Gators links and news we can find. Got a link we should check out? Email us at AlligatorArmy@gmail.com, subject line CAB.

Offseason special airs Sunday: Come for Mick Hubert lobbing softballs to Will Muschamp, stay for Jeff Dillman being a scary human being. (GatorZone)

Matt Elam, Sharrif Floyd on multiple watch lists: Good for them! (GatorZone)

New softball assistant coach is apparently turf maven: I mean, this is really a paragraph in Kenny Gajewski's official hiring announcement. (GatorZone)

Since 2007, Gajewski has been the owner and operator of three Norman, Okla.-based turf business - Champions Athletic Turf (athletic field renovation), Sooner Curb and Border (decorative concrete) and Ameriturf MidSouth (synthetic turf).

Kevin O'Sullivan's cupboard gets more bare: Six Florida signees drafted, six draft picks heading to the majors and skipping college.

As always: Talk about any and all of this and/or anything else you have to say in the comments. Need more Alligator Army in your life? Like AA on Facebook, follow AA on Twitter, and download the SB Nation Android app to take all of our Gators content ? and the rest of SB Nation ? with you on the go.

Source: http://www.alligatorarmy.com/2012/7/14/3158555/florida-gators-offseason-special-sun-sports

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Windows 8 Hackathon

Windows 8 changes everything.
Combining the broad reach of Windows, best-in-class developer tools, a re-imagined user experience, support for new chipsets, and a built-in store with industry-leading business terms, Windows 8 is the largest developer opportunity ? ever.

Our Hackathon is an open Windows 8 code fest, where you?ll put what you know into practice and be eligible to win some great prizes! Code to your heart?s content, with Windows 8 experts available to guide you through every step of the process. It?s the perfect opportunity to get your dream application underway, or to finish that app you?ve already started.

This full-day event will be filled with coding, sharing, plenty of food, and perhaps the occasional Lightning Talk on topics determined by your apps and questions. Bring your PCs installed with (insert download link here), your apps and your cool ideas and get ready to create!

Source: http://www.communitymegaphone.com/ShowEvent.aspx?EventID=5638

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China concludes disabled Tiananmen Square protestor ?committed suicide? - Indiatalkies.com

Beijing, July 14: Chinese police has said that a second investigation into the death of leading dissident Li Wangyang showed that he had committed suicide.

Authorities in the country?s Hunan province said that the conclusion was made in consultation with forensic experts and experienced investigators.

According to the BBC, Wangyang was found dead in his hospital room in the central Chinese city of Shaoyang on June 6.

Wangyang spent more than 22 years in jail after taking part in the Tiananmen Square protests of 1989, and his death sparked off protests in Hong Kong.

Following his death, a senior Hong Kong official said he doubted the nearly deaf and blind activist could hang himself.

According to the report, Chinese authorities claim he had committed suicide while under police guard.

But Wangyang? friends had suggested that the suicide seemed to be staged and accused authorities of rushing to cremate the activist?s body following an autopsy. (ANI)

Source: http://www.indiatalkies.com/2012/07/china-concludes-disabled-tiananmen-square-protestor-committed-suicide.html

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Discovery opens door to attacking biofilms that cause chronic infections

Friday, July 13, 2012

A clever new imaging technique discovered at the University of California, Berkeley, reveals a possible plan of attack for many bacterial diseases, such as cholera, lung infections in cystic fibrosis patients and even chronic sinusitis, that form biofilms that make them resistant to antibiotics.

By devising a new fluorescent labeling strategy and employing super-resolution light microscopy, the researchers were able to examine the structure of sticky plaques called bacterial biofilms that make these infections so tenacious. They also identified genetic targets for potential drugs that could break up the bacterial community and expose the bugs to the killing power of antibiotics.

"Eventually, we want to make these bugs homeless," said lead researcher Veysel Berk, a postdoctoral fellow in the Department of Physics and the California Institute for Quantitative Biosciences (QB3) at UC Berkeley.

Berk and his coauthors, including Nobel laureate Steven Chu, report their findings in the July 13 issue of the journal Science.

"In their natural habitat, 99.9 percent of all bacteria live as a community and attach to surfaces as biofilms; according to the National Institutes of Health, 80 percent of all infections in humans are related to biofilms," Berk said.

The researchers were able to employ new techniques that allowed them to zoom into a street-level view of these biofilms, where they learned "how they grow from a single cell and come together to form rooms and whole buildings," Berk said. "Now, we can come up with a logical approach to discovering how to take down their building, or prevent them from forming the building itself."

Combining super-resolution microscopy with the technique Berk developed, which allows continuous labeling of growing and dividing cells in culture, biologists in many fields will be able to record stop-motion video of "how bacteria build their castles," he said.

In a statement about the paper, Chu said, "Advances in single molecule methods based on optical microscopy have made stunning progress in the past two decades. In particular, 'super-resolution' imaging methods enabled researchers to image structures with unprecedented detail. Veysel Berk led a team that applied these optical methods to continuously image live biofilms on length scales on tens of nanometers to tens of microns. Before this work, these bacterial communities were largely studied in terms of their average composition, appearance and bulk biochemistry. By introducing a new in vivo fluorescence tagging strategy, the molecular and architectural roles of three specific matrix proteins and the extraracellular polysaccharides of a growing V. cholerae bioflim were visualized as series of three-dimensional images. This work has led to new insights into the development of these complex structures and will no doubt pave the way to new approaches to fighting infectious disease and also bacteriological applications in environmental and industrial settings."

Bacteria are not loners

The popular view of bacteria is that they are free-living organisms easily kept in check by antibiotics, Berk said. But scientists now realize that bacteria spend most of their lives in colonies or biofilms, even in the human body. While single bacteria may be susceptible to antibiotics, the films can be 1,000 times more resistant and most can only be removed surgically.

Implants, such as pacemakers, stents and artificial joints, occasionally become infected by bacteria that form biofilms. These biofilm sites periodically shed bacteria ? adventurers, Berk calls them ? which can ignite acute infections and fever. While antibiotics can knock out these free-swimming bacteria and temporally calm down the infection, the biofilm remains untouched. The only permanent solution is removal of the biofilm-coated device and replacement with a new sterilized implant.

A permanent bacterial biofilm in the sinuses can ignite an immune response leading to chronic sinus infections, with symptoms including fever and cold-like symptoms. So far, the most effective treatment is to surgically remove the affected tissue.

Bacteria also form permanent, mostly lifelong, biofilms in the mucus-filled lungs of cystic fibrosis patients and are responsible for the chronic lung infections that lead to early death. Although long-lasting antibiotic treatment helps, it cannot eradicate the infection completely.

To study a biofilm formed by cholera bacteria, Berk built his own super-resolution microscope in the basement of UC Berkeley's Stanley Hall based on a 2007 design by coauthor Xiaowei Zhuang, Chu's former post-doctoral student who is now a professor at Harvard University. To actually see these cells as they divided to form "castles," Berk devised a new technique called continuous immunostaining that allowed him to track four separate target molecules by means of four separate fluorescent dyes.

He discovered that, over a period of about six hours, a single bacterium laid down a protein glue to attach itself to a surface, then divided into daughter cells while secreting a protein that cements the daughters cells to one another. The daughters continued to divide until they formed a cluster ? like a brick and mortar building ? at which point the bacteria secreted a protein that, along with sugar molecules, encased the cluster like the shell of a building.

The clusters are separated by microchannels that may allow nutrients in and waste out, Berk said.

"If we can find a drug to get rid of the glue protein, we can move the building as a whole. Or if we can get rid of the cement protein, we can dissolve everything and collapse the building, providing antibiotic access," Berk said. "These can be targets for site-specific, antibiotic medicines in the future."

Super-resolution microscopy: painting with light

Berk is a biologist trained in physics and optics with expertise in imaging the structures of proteins: He was part of a team that a few years ago determined the atomic-scale structures of the ribosome, the cellular machine that translates genetic message into a finished protein.

He suspected that powerful new super-resolution light microscopy could reveal the unknown structure of biofilms. Super-resolution microscopy obtains 10 times better resolution than standard light microscopy ? 20 instead of 200 nanometers ? by highlighting only part of the image at a time using photo-switchable probes and compiling thousands of images into a single snapshot. The process is much like painting with light ? shining a flashlight beam on a dark scene while leaving the camera shutter open. Each snapshot may take a few minutes to compile, but for slow cellular growth, that's quick enough to obtain a stop-action movie.

The problem was how to label the cells with fluorescent dyes to continuously monitor their growth and division. Normally, biologists attach primary antibodies to cells, then flood the cells with fluorescent dye attached to a secondary antibody that latches onto the primary. They then flush away the excess dye, shine light on the dyed cells and photograph the fluorescence.

Berk suspected that a critically balanced concentration of fluorescent stain ? low enough to prevent background, but high enough to have efficient staining ? would work just as well and eliminate the need to flush out excess dye for fear it would create a background glow.

"The classical approach is first staining, then destaining, then taking only a single snapshot," Berk said. "We found a way to do staining and keep all the fluorescent probes inside the solution while we do the imaging, so we can continuously monitor everything, starting from a single cell all the way to a mature biofilm. Instead of one snapshot, we are recording a whole movie."

"It was a very simple, cool idea, but everyone thought it was crazy," he said. "Yes, it was crazy, but it worked."

###

University of California - Berkeley: http://www.berkeley.edu

Thanks to University of California - Berkeley for this article.

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Source: http://www.labspaces.net/121723/Discovery_opens_door_to_attacking_biofilms_that_cause_chronic_infections

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